Jozef J. Bujarski, Ph.D.

Distinguished Research Professor

Education

• Ph.D. 1987 A. Mickiewicz University, Poznan, Poland
• M.S. (Biology) 1976 A. Mickiewicz University, Poznan, Poland
• M.S. (Chemistry) 1972 A. Mickiewicz University, Poznan, Poland

Field of Interest

• Plant molecular biology
• Molecular virology

Research

Study of the mechanisms of RNA-RNA recombination (homologous and non-homologous) that occur in RNA viruses by using Brome Mosaic Bromovirus (BMV) as a model system, from both the virus and the host stand points. The main subjects under study include the determination of viral RNA sequences supporting crossover events, the role of viral replicase (RdRp) complex in recombination and the involvement of host cellular genes and the cellular pathways (such as RNAi/PTGS) in recombination. Also, evaluation of the risk of recombination associated with the use of transgenic plants that carry viral transgenes is another area of interest.

Selected Publications

• Beivy Kolondam, Parth Rao, Joanna Sztuba-Solinska, Philipp H. Weber, Aleksandra Dzianott, Mitrick A. Johns, Jozef J. Bujarski. 2015. Co-infection with two strains of Brome mosaic bromovirus reveals common RNA recombination sites in different hosts. Virus Evol. 1 (1).
• Weber PH, Bujarski JJ. 2015. Multiple functions of capsid proteins in (+) stranded RNA viruses during plant-virus interactions. Virus Res. 196C:140-149.
• Otulak K, Chouda M, Bujarski J, Garbaczewska G. 2014. The evidence of Tobacco rattle virus impact on host plant organelles ultrastructure. Micron. 70C:7-20. doi: 10.1016/j.micron.2014.11.007.
• Bujarski JJ. (2013). Genetic recombination in plant-infecting messenger-sense RNA viruses: overview and research perspectives. Front Plant Sci. 4:68. Epub 2013 Mar 26.
• Dzianott, A., Sztuba-Solinska, J., and Jozef J. Bujarski. 2012. Mutations in the antiviral RNAi defense pathway modify Brome Mosaic Bromovirus RNA Recombinant Profiles. Molecular Plant-Microbe Interactions. 25(1):97-106.
• Olszewska M, Bujarski JJ, Kurpisz M. 2012. P-bodies and their functions during mRNA cell cycle: mini-review. Cell Biochem Funct. 30(3):177-82.
• Poudel B, Sabanadzovic S, Bujarski J, Tzanetakis IE. 2012. Population structure of Blackberry yellow vein associated virus, an emerging crinivirus. Virus Res. 2012 Oct;169(1):272-5.
• Sztuba-Solinska J, Fanning SW, Horn JR, and Bujarski JJ. 2012. Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus. Virus Res. 170(1-2):138-49.
• Aleksandra Dzianott, Joanna Sztuba-Solinska, Anni Moore, and Jozef J. Bujarski. Null alleles of the RNA Interference Genes Affect RNA-RNA Recombination of Brome Mosaic bromovirus in Arabidopsis thaliana. Submitted to Molecular Plant-Microbe Interactions.
• Sztuba-Solinska J, Urbanowicz A, Figlerowicz M, Bujarski JJ. RNA-RNA 2011. Recombination in Plant Virus Replication and Evolution. Annu Rev Phytopathol. Vol. 49.
• Sztuba-Solinska J, Stollar V, Bujarski JJ. 2011. Subgenomic messenger RNAs: mastering regulation of (+)-strand RNA virus life cycle. Virology. 412(2):245-55
• Sztuba-Solinska J, Dzianott A, Bujarski JJ. 2011. Recombination of 5’ subgenomic RNA3a with genomic RNA3 of Brome mosaic bromovirus in vitro and in vivo. Virology. 410(1):129-41.
• Bujarski, J., 2008. BROMOVIRUSES (BROMOVIRIDAE). In R.G. Webster and A. Granoff (eds.), Encyclopaedia of Virology, Third Edition.
  Bujarski, J. 2007. Genetic Recombination in Viruses. Review. Encyclopedia of Virology. 3rd Edition. 2008.
• Bujarski, J. 2007. Bromoviridae. Review. Encyclopedia of Virology. 3rd Edition. 2008
  Sztuba-Solinska J., Bujarski J.J. 2008. Insights into the single-cell reproduction cycle of members of the family bromoviridae: lessons from the use of protoplast systems. J Virol. 82:10330-40.
• Sandoval C, Pogany J, Bujarski J., Romero J. 2008. Use of a defective RNA of broad bean mottle bromovirus for stable gene expression in legumes. Arch Virol. 153:1755-8.
• Wierzchoslawski, R. and Bujarski, J.J. 2006. Efficient in vitro system of homologous recombination in brome mosaic bromovirus. J Virol. 80:6182-7.
• Wierzchoslawski, R., Urbanowicz, A., Dzianott, A., Figlerowicz, M., and Bujarski, J.J. 2006. Characterization of a novel 5' subgenomic RNA3a derived from RNA3 of Brome mosaic bromovirus. J Virol. 80:12357-66.
• Bujarski J. and Wierzchoslawski R. 2006. METHOD OF PRODUCTION AND APPLICATION OF THE RNA POLYMERASE PROTEIN FROM BROME MOSAIC BROMOVIRUS. Patent application.
• Urbanowicz A, Alejska M, Formanowicz P, Blazewicz J, Figlerowicz M, Bujarski JJ. 2005.Homologous crossovers among molecules of brome mosaic bromovirus RNA1 or RNA2 segments in vivo. Journal of Virology 79:5732-42.
• Wierzchoslawski R, Bujarski JJ. 2004. Efficient in vitro system of homologous recombination in brome mosaic bromovirus. J Virol. 80:6182-7.