Linda S. Yasui, Ph.D.

Distinguished Research Professor

Education

• Ph.D., 1984, Florida State University
• M.A., 1979, Florida State University
• A.B., 1977, University of California, Berkeley

Field of Interest

• Radiation biology
• DNA damage and repair in chromatin

Research

We are interested in finding out how radiation kills cancer cells by using high LET radiation sources such as 125I decay, fast neutrons and thermal neutrons. Our recent projects include investigations aimed at looking at chromatin damage by 125I decay and neutron irradiation of human breast cancer cells, brain cancer cells and ovarian cancer cells. We are especially interested in investigating epigenetic mechanisms for cell death that quantify DNA double strand breaks (as detected by the formation of gH2AX foci imaged by confocal laser scanning microscopy) as they relate to apoptosis versus autophagy in human cancer cells.

Representative Publications

• P Vodnala, N Karunaratne, S Bera, L Lurio, GM Thurston, N Karonis, J Winans, A Sandy, S Narayanan, L Yasui, E Gaillard, K Karumanchi. 2015. Radiation Damage Limits to XPCS Studies of Protein
• Dynamics. 12th International Conference on Synchrotron Radiation Instrumentation (SRI 2015). AIP Conference Proceedings (In press)
• Klionsky et al., Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy (2^nd edition). Autophagy (in prep)
• LS Yasui, M Johnson, H Savage, C Burns, A Baker, E Baker. 2015. Measurement of radiation-induced autophagic flux in glioblastoma multiforme cells. Microscopy and Microanalysis. In press
• LS Yasui, M Duran, C Andorf, T Kroc, K Owens, A Schuck, K Allen, R Becker, WS Grayburn. 2015. Metabolic heterogeneity in glioblastoma cells. International Journal of Radiation Biology. Submitted
• LS Yasui, K Owens, M Foster, W Miter. Radiation-induced necrosis in glioblastoma multiforme compared to normal human astrocytes. In prep.
• LS Yasui, ML Foster. Radiation-induced cell death by cisplatin combined with g or fast neutron irradiation in glioblastoma multiforme cells. International Journal of Radiation Biology. Submitted
• LS Yasui, WS Grayburn, C Andorf, T Kroc, A Lennox. Mixed modes of cell death in fast neutron irradiated glioblastoma multiforme cells. Submitted.
• Yasui, L. (2013). Imaging cytometry of radiation-induced translocation of nuclear HMGB1 across the cell. Microscopy and Microanalysis, 19 (suppl 2), 264-265.
• Yasui, L. (2012). Molecular and cellular effects of Auger emitters: 2008-2011. International Journal of Radiation Biology, 88, 864-870.
• Yasui, L., Owens, K. (2012). Necrosis induction by gadolinium neutron capture in glioblastoma multiforme cells.. International Journal of Radiation Biology, 88, 980-990.
• Yasui, L., Kroc, T., Gladden, S., Andorf, C., Bux, S., Hosmane, N. (2012). Boron neutron capture in prostate cancer cells. Applied Radiation and Isotopes, 70, 6-12.
• Yasui, L., Gladden, S., Andorf, C., Kroc, T., Lennox, A. (2011). Fast neutron induced autophagy leading to necrosis in glioblastoma multiforme.. Applications of Accelerators in Research and Industry. AIP Conference Proceedings, 1336, 377-382.
• LS Yasui, T Kroc, C Andorf, L Schneider, A Lennox, KR Saroja. 2008. Gadolinium neutron capture in glioblastoma multiforme cells. International Journal of Radiation Biology. 84, 1130-1139.
• DP Lotshaw, WS Grayburn, LS Yasui, KW Gasser. 2008. Expression of the two-pore domain potassium channel, TASK-2, in rat pancreatic acinar cell zymogen granule membrane. FASEB J 22, 938.
• R.W. Howell, R.F. Martin, H. Nikjoo, E. Pomplun, M. Terrissol, R. Watanabe, L. Yasui, A. Kassis, S.J. Adelstein. 2008. Meeting overview. International Journal of Radiation Biology 84, 957-958.
• RN Bose, L. Maurmann, R.J. Mishur, L.S. Yasui, S. Gupta, W.S. Grayburn, H. Hofstetter. 2008 Novel activities of non-DNA binding platinum(II)- and platinum(IV)-phophato complexes towards human ovarian cells. Proceedings of the National Academy of Sciences USA 1-5, 18314-18319.
• Yasui, L.S., Chen, K., Jones, T.P., Caldwell, J., Guse, D., Kassis, A.I. 2007. Using Hoeschst 33342 to target radioactivity to the cell nucleus. Radiation Research 167, 167-175.
• Yasui, L. S. 2006 Imaging radiation induced yH2ax foci. Microscopy and Microanalysis 12, 248 CD. P. Kotula, M. Marko, J.H. Scott, R. Gavin, D. Bariac, C. Lukas, S. McKennan, J. Shields, eds. Cambridge University Press.
• Mork, K., Jones, T.P., Yasui, L.S. 2005. DNA double strand break detection. In, Updates in Science and Technology. Abstract published as part of the Joint meeting of the 16th Annual Argonne Symposium for Undergraduates in Science, Engineering and Mathematics and The Central States Universities, Inc. (CSUI).
• Yasui, L. S. 2004. gH2AX foci induced by g rays and 125IdU decay. International Journal of Radiation Biology 80: 895-903.
• Yasui, L.S., A. Hughes, E.R. DeSombre. 2001. Cytoxicity of 125I estrogen decay in non-oestrogen expressing human breast cancer cellls, MDA-231 and Oestrogen receptor expressing MCF-7 cells. International Journal of Radiation Biology. 77, 955-962.
• Yasui, L.S., A. Hughes, E.R. DeSombre 2001. Relative biological effectiveness of accumulated 125IUdR and 125I estrogen decays in estrogen receptor expressing human breast cancer cells. Radiation Research 55:328-334.
• Yasui, L.S., A. Hughes, E.R. DeSombre. 2000. Production of clustered DNA damage by 125I decay. Acta Oncologica 39:739-740.

Abstracts

• L.S. Yasui, A. Hughes and E.R. DeSombre. 1995. DNA damage induction by 125I-estrogen. presented by Eugene DeSombre at the Third International Symposium on Biophysical Aspects of Auger Processes. University of Lund, Lund, Sweden. Aug. 24-25, 1995.
• L.S. Yasui, L. Fink, M. Lin and A.M. Enrique. 1995. Repair of 125I decay induced DNA damage in CHO K1 and xrs-5 cells. Poster at the Third International Symposium on Biophysical Aspects of Auger Processes. University of Lund, Lund, Sweden. Aug. 24-25, 1995.
• L.S. Yasui, D. Ferns, B. Dabbah, a. Hughes, E. DeSombre. 1996. 125I decay sensitivity in estrogen receptor expressing CHOER cells. Radiation Research meeting. Chicago, IL. Apr. 14-17, 1996.
• L.S. Yasui and J.L. Schwartz. 1997. Detection of Ku p70 and p80 in CHO K1 and xrs-5 cells. Radiation Research meeting. Providence, R.I. May 3-7, 1997.