MALDI

MALDIMatrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry for the non-chromatographic analysis of biopolymers (e.g, proteins, peptides and carbohydrates) and large organic molecules (e.g, polymers, dendrimers). Analytes are embedded in a matrix which absorbs energy at the wavelength of the laser to facilitate vaporization and ionization.

The AB Voyager-DE BioSpectrometry Workstation is a matrix-assisted laser desorption time-of-flight mass spectrometer (MALDI-TOF MS) with a mass range of 500 to approximately 300,000 Da. It is equipped with a pulsed ultraviolet nitrogen laser (337nm, 3 ns pulse, 20 Hz maximum firing rate), a time-of-flight mass analyzer with a 1.2 m linear flight path. The instrument can be operated in delayed extraction mode.

Data acquisition and processing is controlled by the Voyager Control Panel and spectra can be analyzed using DataExplorer.

To access the MALDI, users must set up an account. Please contact Dr. Taesam Kim for more information.


MALDI

Figure 1. MALDI spectrum of a BSA digest after cleaning with C18 Ziptip