Core Microscopy Facilitiy

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The Core Microscopy Facility (CMF) assists faculty and student researchers with projects that utilize advanced microscopy equipment and techniques. In addition to housing the Biology Departments specialized research microscopes, the CMF maintains equipment and supplies for preparation of specimens for electron, transmitted light, and confocal microscopic analysis.

The CMF is home to a Zeiss LSM 5 Pascal Confocal Laser Scanning Microscope (CLSM) (above) and a Hitachi H-600 Transmission Electron Microscope (TEM) (right).

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Imaging Equipment:

Hitachi H-600 Transmission Electron Microscope (TEM)

  • SIA L12C (16 mp) imaging system with MaxIm DL 5 image capture and optimization software

Nikon Eclipse E-600 Research Light Microscope (LM) outfitted with:

  • Standard Brightfield visualization
  • Epifluorescence filter sets:
  • Differential Interference Contrast (DIC, a.k.a. Nomarski optics)
  • Phase Contrast
  • 1X, 4x, 10x, 40x, and 100x (oil) Plan Fluor,and 60x Plan Apo objectives
  • Nikon DS-Fi1c color digital camera system with Nikon NIS-Elements image capture and optimization software
  • Hamamatsu ORCA-ER high-sensitivity monochrome digital CCD camera with HCImage Live image capture and optimization software

Zeiss LSM 5 Pascal Confocal Laser Scanning Microscope outfitted with:

  • 10x, 40x (oil), and 63x (oil) Plan Neofluar objectives
  • Argon (458, 488, and 514 nm) and HeNe (543 nm) lasers
  • Zeiss Pascal LSM 5 image acquisition and optimization software

Beckman Coulter Cell Lab Quanta SC Flow Cytometer

  • Argon 488 nm laser
  • Mercury Arc Lamp

Leica MZ7.5 Stereo Microscope 

  • Leica MC170 HD color digital camera
  • Leica Application Suite image capture and optimization software

Equipment Training Resources

Sample Preparation Resources:

Preparation Equipment:

For Transmission Electron Microscopy:

  • Reichert-Jung SuperNova Ultramicrotome
  • Reichert OmU2 Ultramicrotome
  • Sorvall Porter-Blum MT2-B Ultramicrotomes (2)
  • LKB Glass Knifemaker
  • Leica EMStain Autostainer

For Light Microscopy:

  • AO T/P 8000 Automated Tissue Processor with Tissue-Tek II Paraffin Dispenser
  • AO Spencer 820 Microtomes (3)
  • Lab-Line Slide Warmer
  • Lab-Line and Tissue-Tek tissue flotation baths
  • AO Knife Sharpener

Services:

  • Training in tissue preparation and equipment usage
  • Class demonstrations & tours
  • Fixation, embedding, sectioning, and staining for TEM and LM applications
  • Assistance with immunohistochemical preparations
  • Assistance with image acquisition and optimization
  • TEM negative development and scanning (using Epson Perfection 2450 Photo scanner