The Department of Chemistry and Biochemistry
The Michael Faraday Laboratories
Northern Illinois University
DeKalb, IL 60115-2862
Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry for the non-chromatographic analysis of biopolymers (e.g, proteins, peptides and carbohydrates) and large organic molecules (e.g, polymers, dendrimers). Analytes are embedded in a matrix which absorbs energy at the wavelength of the laser to facilitate vaporization and ionization.
The AB Voyager-DE BioSpectrometry Workstation is a matrix-assisted laser desorption time-of-flight mass spectrometer (MALDI-TOF MS) with a mass range of 500 to approximately 300,000 Da. It is equipped with a pulsed ultraviolet nitrogen laser (337nm, 3 ns pulse, 20 Hz maximum firing rate), a time-of-flight mass analyzer with a 1.2 m linear flight path. The instrument can be operated in delayed extraction mode.
Data acquisition and processing is controlled by the Voyager Control Panel and spectra can be analyzed using DataExplorer.
- Voyager Getting Started Guide (PDF)
- Voyager User's Guide (PDF)
- Data Explorer User's Guide (PDF)
- MALDI Info by John Innes Centre
- MALDI Matrices by Sigma-Aldrich (PDF)
- MALDI Matrices by Pierce (PDF)
To access the MALDI, users must set up an account. Please contact Dr. Taesam Kim for more information.
Figure 1. MALDI spectrum of a BSA digest after cleaning with C18 Ziptip